bullet Sensors & Transducers Journal

    (ISSN 1726- 5479)

205.767

2008 e-Impact Factor

25 Top Downloaded Articles

Journal Subscription 2012

Editorial Calendar 2012

Submit an Article

Editorial Board

Current Issue

Sensors & Transducers journal's cover

Sensors & Transducers Journal 2011

Sensors & Transducers Journal 2010

Sensors & Transducers Journal 2009

Sensors & Transducers Journal 2008

Sensors & Transducers Journal 2007

2000-2002 S&T e-Digest Contents

2003 S&T e-Digest Contents

2004 S&T e-Digest Contents

2005 S&T e-Digest Contents

2006 S&T e-Digest Contents

 

Best Articles 2011

 

 

 

Vol. 143, No. 8, August 2012, pp. 152-161

 

Bullet

 

Comparison Sensitivity of Acetylcholinesterase Biosensor based on Different Electrode-modified Methods
 
a, b Wenping ZHAO, b Xia SUN, b Chen ZHAI, b Xiangyou WANG, a Zhihuai MAO

a College of Engineering, China Agricultural University, 17 Qinghua Donglu, Beijing 100083, China

Tel.: +86-010-62737351, fax: +86-010- 62737351

b School of Agriculture and Food Engineering, Shandong University of Technology, No.12,
Zhangzhou Road, Zibo 255049, Shandong Province, China

Tel.: +86-533-2786558, fax: +86-533-2786558

E-mail: Maozhh@bjaeu.edu.cn, xiasun2151@sina.com, wxy@sdut.edu.cn

 

Received: 23 May 2012   /Accepted: 21 August 2012   /Published: 28 August 2012

Digital Sensors and Sensor Sysstems

 

Abstract: Based on the change in electrochemical behavior of enzymatic activity, six kinds of acetylcholinesterase biosensor have been devised for investigation of electrodes sensitivity using different modified methods. Electrochemical impedance spectroscopy (EIS) and cyclic voltammograms (CV) were used to investigate the electrochemical response of the six different modified glassy carbon electrodes, respectively. The results shown that the gelatinous composite containing functional multiwall carbon nanotubes (FCNTs), glutaraldehyde (GTA) and chitosan (CS) was the best candidate to modify the GCE. Because of the excellent biocompatibility and good stability of chitosan matrix, it prevented leakage of the AChE from electrode. FCNTs promoted electron transfer reaction at a lower potential and catalyzed the electro-oxidation of thiocholine, thus amplifying the sensitivity and amperometric response of the biosensor. The peak current of FCNTs-GTA-CS/GCE biosensor can reach up to 140 μA and the peak voltage low to 600 mV. The developed biosensor exhibited good reproducibility, acceptable stability, fast response and low detection limit, thus providing a new promising tool for biosensor design.

 

Keywords: Biosensor, Acetylcholinesterase, Functional multiwall carbon nanotubes, Prussian blue, Electrode modification

 

 

Buy this article online or subscribe Sensors & Transducers journal (12 issues per year plus special issues; 40 % discount for payment IFSA Members):

 

 

Sensors & Transducers journal subscription

450 $ US per year:

 

Buy this review article for
14.95 $ US:

 

 
 

 

 
 

Alternatively we accept a money transfer to our bank account. Please contact for details: sales@sensorsportal.com

 

Download <here> the Library Journal Recommendation Form

 

 

Read more about Biosensors Sensors

 

 

 

 

 


1999 - 2012 Copyright , International Frequency Sensor Association (IFSA). All Rights Reserved.


Home - News - Links - Archives - Tools - Voltage-to-Frequency Converters - Standardization - Patents - Marketplace - Projects - Wish List - e-Shop - Sensor Jobs - Membership - Videos - Publishing - Site Map - Subscribe - Search

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Submit Press Release - Submit White Paper - Testimonies - Twitter - Facebook - LinkedIn