bullet Sensors & Transducers Journal

    (ISSN 1726- 5479)

205.767

2008 e-Impact Factor

25 Top Downloaded Articles

Best Selling Articles 2012

Journal Subscription 2013

Editorial Calendar 2012

Submit an Article

Editorial Board

Current Issue

Sensors & Transducers book's cover

Sensors & Transducers Journal 2011

Sensors & Transducers Journal 2010

Sensors & Transducers Journal 2009

Sensors & Transducers Journal 2008

Sensors & Transducers Journal 2007

2000-2002 S&T e-Digest Contents

2003 S&T e-Digest Contents

2004 S&T e-Digest Contents

2005 S&T e-Digest Contents

2006 S&T e-Digest Contents

 

Best Articles 2011

 

 

 

Vol. 144, No. 9, September 2012, pp. 153-160

 

Bullet

 

Quartz Crystal Microbalance DNA Based Biosensor for the Detection of Brugia malayi
 

1 Thongchai KAEWPHINIT, 2 Somchai SANTIWATANAKUL, 3 Supatra AREEKIT and 4 Kosum CHANSIRI

1 Graduate School of Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand

Tel.: +66 22664-1000 ext 4619

2 Department of Pathology, Faculty of Medicine, Srinakharinwirot University,
Sukhumvit 23, Bangkok, 10110, Thailand

3 Innovative Learning Center, Srinakharinwirot University,
Sukhumvit 23, Bangkok, 10110, Thailand

4 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University,
 Sukhumvit 23, Bangkok, 10110, Thailand

Tel.: +66 2260-2122 ext 4605, fax: +66 2260-0125

E-mail: tkaewphinit@yahoo.com, titi41@yahoo.com, jeedkha@hotmail.com, kchansiri@yahoo.com

 

 

Received: 2012   /Accepted: 21 September 2012   /Published: 28 September 2012

Digital Sensors and Sensor Sysstems

 

Abstract: This Lymphatic filariasis is the major public health problem caused by Brugia spp. by the conventional methods as PCR cannot discriminate well, takes a few days or several hours. This study demonstrates a sensitive and specific quartz crystal microbalance (QCM) biosensor combined with polymerase chain reaction (PCR) for diagnosis of Brugia malayi. The procedure concludes: formation of self-assembled monolayer (SAM) on gold quartz crystals surface, attachment of the avidin to activated carboxyl groups by EDC/NHS, attachment of the biotin-modified probe to the avidin, and hybridization of probe to the target PCR. The PCR-QCM biosensor system was more specific than a PCR-gel electrophoresis assay in detecting the DNA of B. malayi and B. pahangi.

 

Keywords: QCM, DNA immobilization sensing, Lymphatic filariasis, B. malayi

 

 

Buy this article online (it will be send to you in the pdf format by e-mail) or subscribe Sensors & Transducers journal

(12 issues per year plus special issues; 40 % discount for payment IFSA Members):

 

 

Sensors & Transducers journal subscription

450 $ US per year:

 

Buy this article for
14.95 $ US:

 

 
 

 

 
 

Alternatively we accept a money transfer to our bank account. Please contact for details: sales@sensorsportal.com

 

Download <here> the Library Journal Recommendation Form

 

 

Read more about Biosensors

 

 

 

 

 


1999 - 2012 Copyright , International Frequency Sensor Association (IFSA). All Rights Reserved.


Home - News - Links - Archives - Tools - Voltage-to-Frequency Converters - Standardization - Patents - Marketplace - Projects - Wish List - e-Shop - Sensor Jobs - Membership - Videos - Publishing - Site Map - Subscribe - Search

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Submit Press Release - Submit White Paper - Testimonies - Twitter - Facebook - LinkedIn