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Vol. 146, No. 11, November 2012, pp. 78-89

 

Bullet

 

Amperometric Acetylcholinesterase Biosensor Based on Poly (Diallyldimethylammonium Chloride)/Gold Nanoparticles/Multi-walled Carbon Nanotubes-chitosans Composite Film-modified Electrode
 
* Xia Sun, Zhili Gong, Yaoyao Cao, * Xiangyou Wang

School of Agriculture and Food Engineering, Shandong University of Technology,
No. 12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China

Tel.:+86-533-2786558, fax: +86-533-2786558

E-mail: sunxia2151@sina.com, wxy@sdut.edu.cn

 

Received: 26 September 2012   /Accepted: 23 November 2012   /Published: 30 November 2012

Digital Sensors and Sensor Sysstems

 

Abstract: A novel amperometric biosensor was developed for the detection of organophosphorus pesticides (OPs). Acetylcholineesterase (AChE) was immobilized onto poly(diallyldimethylammonium chloride) (PDDA)/gold nanoparticles (AuNPs)/multi-walled carbon nanotubes-chitosans (MWCNTs-CHIT) composite film modified glass carbon electrode (GCE). The biosensor had good sensitivity owing to the excellent properties of MWCNTs and AuNPs, which promoted electron transfer and improved the electrochemical response. PDDA with positive charge was bound electrostatically to AChE with negatively charge by layer-by-layer (LbL) self-assembly technique, which can increase AChE loading amount. The morphologies and the sensitive steps of surface modification were characterized by scanning electron microscopy (SEM), cyclic voltammetric (CV) and electrochemical impedance spectroscopy (EIS), respectively. Based on the inhibition of OPs on the AChE activity, under the optimized conditions, the biosensor exhibited a linear response to chlorpyrifos in the concentration range from 0.8 to 20 μg/L and from 20 to 300 μg/L, with a detection limit of 0.56 g/L. The biosensor exhibited good reproducibility and acceptable stability. Moreover, PDDA-AChE/AuNPs/MWCNTs- CHIT/GCE biosensor can also be used for direct analysis of practical samples, which may open a new door for pesticide analysis in vegetables and fruits.

 

Keywords: Acetylcholinesterase, Multiwall carbon nanotubes, Gold nanoparticles, Poly (diallyldimethylammonium chloride), Biosensor

 

 

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