bullet Sensors & Transducers Journal

    (ISSN 1726- 5479)

205.767

2008 e-Impact Factor

25 Top Downloaded Articles

Best Selling Articles 2012

Journal Subscription 2013

Editorial Calendar 2013

Submit an Article

Editorial Board

Current Issue

Sensors & Transducers journal's cover

Sensors & Transducers Journal 2011

Sensors & Transducers Journal 2010

Sensors & Transducers Journal 2009

Sensors & Transducers Journal 2008

Sensors & Transducers Journal 2007

2000-2002 S&T e-Digest Contents

2003 S&T e-Digest Contents

2004 S&T e-Digest Contents

2005 S&T e-Digest Contents

2006 S&T e-Digest Contents

 

Best Articles 2011

 

 

 

Vol. 146, No. 11, November 2012, pp. 96-109

 

Bullet

 

A Novel Amperometric Immunosensor Based on {MWCNTs-COOH-CHIT}2/GNPs for Detection of Chlorpyrifos
 
Xia SUN, Lu QIAO, Xiangyou WANG

School of Agriculture and Food Engineering,

Shandong University of Technology,
No.12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China

Tel.:+86-533-2786558, fax: +86-533-2786558

E-mail: sunxia2151@sina.com, wxy@sdut.edu.cn

 

Received: 16 September 2012   /Accepted: 23 November 2012   /Published: 30 November 2012

Digital Sensors and Sensor Sysstems

 

Abstract: In this study, a novel label-free amperometric immunosensor based on gold nanoparticles and dual-layer carboxylated multiwall carbon nanotubes-chitosan ({MWCNTs-COOH-CHIT}2/GNPs) for the determination of chlorpyrifos residues was exploited. To construct the immunosensor, MWCNTs-COOH was first dispersed with CHIT to obtain a homogeneous solution and then it was dropped on the surface of glassy carbon electrode (GCE) which was first modified by the electrodeposition of GNPs. And then the modified GCE was immersed in the poly (diallyldimethylammonium chloride) (PDDA) which was positively charged. Next, the negatively charged MWCNTs-COOH-CHIT was assembled to the interface through the electrostatic force. The stepwise assembly process of electroactive species on electrode surface and the performance of the immunosensor was characterized by means of cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM), respectively. {MWCNTs-COOH-CHIT}2 was acted as an electron promoter and had excellent immobilization effects for antibody, thus, it improved the detection sensitivity and stability of the immunosensor. Under optimal experimental conditions, the proposed immunosensor had a wide linear range from 0.1 to 50 ng/mL and from 50ng/mL to 1mg/mL, and with a detection limit of 0.069 ng/mL. The proposed immunosensor exhibited high reproducibility, specificity, stability and regeneration performance, which provided a new promising tool for the detection of chlorpyrifos residues in fruits and vegetables.

 

Keywords: Amperometric immunosensor, Gold nanoparticles, MWCNTs-COOH-CHIT, Chlorpyrifos

 

 

Buy this article online (it will be send to you in the pdf format by e-mail) or subscribe Sensors & Transducers journal

(12 issues per year plus special issues; 40 % discount for payment IFSA Members):

 

 

Sensors & Transducers journal subscription

450 $ US per year:

 

Buy this article for
14.95 $ US:

 

 
 

 

 
 

Alternatively we accept a money transfer to our bank account. Please contact for details: sales@sensorsportal.com

 

Download <here> the Library Journal Recommendation Form

 

 

 

Read more about Nanosensors and Biosensors

 

 

 

 

 


1999 - 2012 Copyright , International Frequency Sensor Association (IFSA). All Rights Reserved.


Home - News - Links - Archives - Tools - Voltage-to-Frequency Converters - Standardization - Patents - Marketplace - Projects - Wish List - e-Shop - Sensor Jobs - Membership - Videos - Publishing - Site Map - Subscribe - Search

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Submit Press Release - Submit White Paper - Testimonies - Twitter - Facebook - LinkedIn