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Vol. 149, No. 2, February 2013, pp. 123-128

 

Bullet

 

Colorimetric DNA Based Biosensor Combined with Loop-mediated Isothermal Amplification for Detection of Mycobacterium Tuberculosis
by Using Gold Nanoprobe Aggregation
 

1 Thongchai KAEWPHINIT, 2 Somchai SANTIWATANAKUL and 1 Kosum CHANSIRI

1 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University,

Sukhumvit 23, Bangkok, 10110, Thailand

Tel.: +66 2260-2122 ext 4605, fax: +66 2260-0125.

2 Department of Pathology, Faculty of Medicine, Srinakharinwirot University,

Sukhumvit 23, Bangkok, 10110, Thailand

E-mail: tkaewphinit@yahoo.com, kosum@swu.ac.th, titi41@yahoo.com

 

Received: 4 November 2013   /Accepted: 14 February 2013   /Published: 28 February 2013

Digital Sensors and Sensor Sysstems

 

Abstract: Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here, a LAMP method was combined with a gold nanoparticle (AuNPs) probes to detect IS6110 of M. tuberculosis rapidly, and specifically. The reaction amplified DNA and hybridized to thiol modified oligonucleotide probe for 5 min was detected at AuNPs color change 5 min after application. Excluding for the step of DNA extraction, test results could be generated approximately within 1 h. Furthermore, the data indicated that LAMP-AuNPs could detect serial dilution of M. tuberculosis DNA limited as 5 pg of genomic DNA. According to the sensitivity, specificity, less time consuming, low cost and convenience, this technique may prove to be a powerful tool for the early diagnosis of M. tuberculosis.

 

Keywords: Loop-mediated isothermal amplification; LAMP; Gold nanopaticles; AuNPs; Mycobacterium tuberculosis

 

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