bullet Sensors & Transducers Journal

    (ISSN 1726- 5479)


2008 e-Impact Factor

25 Top Downloaded Articles

Best Selling Articles 2012

Journal Subscription 2013

Editorial Calendar 2013

Submit an Article

Editorial Board

Current Issue

Sensors & Transducers journal's cover

Sensors & Transducers Journal 2011

Sensors & Transducers Journal 2010

Sensors & Transducers Journal 2009

Sensors & Transducers Journal 2008

Sensors & Transducers Journal 2007

2000-2002 S&T e-Digest Contents

2003 S&T e-Digest Contents

2004 S&T e-Digest Contents

2005 S&T e-Digest Contents

2006 S&T e-Digest Contents


Best Articles 2011




Vol. 149, No. 2, February 2013, pp. 123-128




Colorimetric DNA Based Biosensor Combined with Loop-mediated Isothermal Amplification for Detection of Mycobacterium Tuberculosis
by Using Gold Nanoprobe Aggregation


1 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University,

Sukhumvit 23, Bangkok, 10110, Thailand

Tel.: +66 2260-2122 ext 4605, fax: +66 2260-0125.

2 Department of Pathology, Faculty of Medicine, Srinakharinwirot University,

Sukhumvit 23, Bangkok, 10110, Thailand

E-mail: tkaewphinit@yahoo.com, kosum@swu.ac.th, titi41@yahoo.com


Received: 4 November 2013   /Accepted: 14 February 2013   /Published: 28 February 2013

Digital Sensors and Sensor Sysstems


Abstract: Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here, a LAMP method was combined with a gold nanoparticle (AuNPs) probes to detect IS6110 of M. tuberculosis rapidly, and specifically. The reaction amplified DNA and hybridized to thiol modified oligonucleotide probe for 5 min was detected at AuNPs color change 5 min after application. Excluding for the step of DNA extraction, test results could be generated approximately within 1 h. Furthermore, the data indicated that LAMP-AuNPs could detect serial dilution of M. tuberculosis DNA limited as 5 pg of genomic DNA. According to the sensitivity, specificity, less time consuming, low cost and convenience, this technique may prove to be a powerful tool for the early diagnosis of M. tuberculosis.


Keywords: Loop-mediated isothermal amplification; LAMP; Gold nanopaticles; AuNPs; Mycobacterium tuberculosis


Acrobat reader logo Click <here> or title of paper to download the full pages article in pdf format



Read free articles about Biosensors






1999 - 2018 Copyright , International Frequency Sensor Association (IFSA). All Rights Reserved.

Home - News - Links - Archives - Tools - Voltage-to-Frequency Converters - Standardization - Patents - Marketplace - Projects - Wish List - e-Shop - Sensor Jobs - Membership - Videos - Publishing - Site Map - Subscribe - Search

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Submit Press Release - Submit White Paper - Testimonies - Twitter - Facebook - LinkedIn