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Vol. 149, No. 2, February 2013, pp. 135-142

 

Bullet

 

Electrochemical Characterization of Enzymatic Impedimetric Biosensor Destined to Detect Organochlorine Pesticide:
the Diclofop-methyl

 

a S. Baali, a S. Zougar, a R. Kherrat, b Z. Djeghaba, b F. Benamia, c N. Jaffrezic-Renault

a Laboratory of Environmental Engineering, Department of Process Engineering,

Faculty of Engineering Sciences, Badji Mokhtar University -Annaba, B. P. 12, Annaba, 23000, Algeria

b Laboratory of Applied Organic Chemistry, Department of Chemistry, B. P. 12, Annaba, 23000, Algeria

c University of Lyon, Laboratory of Analytical Sciences, UMR-CNRS 5180, Claude Bernard University,

Lyon I, 43 Boulevard du 11 November 1918, 69622 Villeurbanne Cedex, France

Tel.: +21307 823 1440, fax: +213 823 3315

E-mail: souadbaali@yahoo.fr, rkherrat@yahoo.fr

 

Received: 2 January 2013   /Accepted: 14 February 2013   /Published: 28 February 2013

Digital Sensors and Sensor Sysstems

 

Abstract: Preservation of aquatic ecosystems requires early warning tools such as biosensors for in situ monitoring and continuously. Such tools can provide information about the nature of pollutants condition be designed to meet specific manner. This work proposes an impedimetric biosensor, using a bacterial enzyme (Condida Rugosa lipase) immobilized in an organic matrix composed of BSA (bovine serum albumin) and glutaraldehyde, capable of detecting organochlorine pesticides (Diclofop-methyl) state trace in aqueous media. Moreover, these measurements were carried out by varying a number of parameters characteristic of the system being studied in order to better define the role of the different elements involved in the development of the receiving part of the sensor as follows: Composition of the membrane, effect of temperature and the effect of the pH of the medium. The results show that the developed biosensor provides answers in an area of very low concentrations of the order of 5.99x10-14 - 5.88x10-3 g/L and a detection limit of 5.99x10-14 g/L for Diclofop-methyl. This biosensor detects the pollutant in a temperature range between 20 C and 40 C. We also studied the selectivity of the detection of the target substance in the presence of interfering with some heavy metals (cadmium, cobalt, zinc and lead) and other organophosphorus and organochlorines pesticide.

 

Keywords: Impedimetric biosensor, Condida Rugosa lipase, Organochlorine pesticide, Dichlofop-methyl, Detection.

 

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