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Vol. 149, No. 2, February 2013, pp. 156-165




Fabrication of an Electrochemical Immunosensor for Carbofuran Detection Based on a Nanocomposite Film

Shuyuan DU, Lu QIAO, * Xiangyou WANG, * Xia SUN

School of Agriculture and Food Engineering, Shandong University of Technology,

NO.12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China

Tel.:+86-533-2786558, fax: +86-533-2786558

E-mail: wxy@sdut.edu.cn, sunxia2151@sina.com


Received: 30 December 2012   /Accepted: 14 February 2013   /Published: 28 February 2013

Digital Sensors and Sensor Sysstems


Abstract: In this study, a sensitive, label-free electrochemical immunosensor for the direct determination of carbofuran was developed. A gold electrode was modified with gold nanoparticles (DpAu), 4,4'-Thiobisbenzenethiol-3-Mercaptopropionic acid-gold colloidal nanoparticles (DMDPSE-MPA-GNPs) nanocomposite film and the antibody (Ab) was orientedly immobilized with protein A (SPA). DpAu was used to enhance the electroactivity and stability of the immunosensor. The porous composite nanocomposite film provided many reactive groups to cross-link immobilize SPA. The formation of a self-assembled SPA layer was employed to the electrode surface to increase the binding capacity of antibody. The conformational properties of the immunosensor and electrochemical performance of immunoreaction between carbofuran and the anti-carbofuran monoclonal antibody (Ab) were characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and differential pulse voltammetric (DPV), respectively. Effects of experimental variables, such as the adsorption time of DpAu, the concentration of antibody solution, the pH of supporting electrolyte and the incubation time were investigated in details. Under optimum operating conditions, the immunosensor provided a wide linear range between 0.1 ng/mL and 10 μg/mL with a low detection limit of 0.0068 ng/mL. The proposed immunosensor exhibited good reproducibility and stability, and it would be a new promising tool for pesticide analysis in food samples and environments.


Keywords: Amperometric immunosensor, Gold nanoparticles, Composite nanocomposit film, Protein A, Carbofuran


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