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Vol. 149, No. 2, February 2013, pp. 179-186

 

Bullet

 

Detection of Ionization Radiation Effect Using Microorganism (Escherichia Coli)

 

1, 3 Maytham Al- Shanawa, 1 A. Nabok, 1* A. Hashim, 2 T. Smith

1 Material and Engineering Research Institute (MERI), Sheffield Hallam University, UK

2 Biomedical Research Centre (BMRC), Sheffield Hallam University, UK

3 Faculty of Science, University of Basra, Iraq

Tel.:+44 1142256905

E-mail: a.hashim@shu.ac.uk

 

Received: 25 December 2012   /Accepted: 14 February 2013   /Published: 28 February 2013

Digital Sensors and Sensor Sysstems

 

Abstract: Thousands of waste sites around the world contained different pollutants and toxins, particularly heavy metals and radioactive elements. The most dangerous type of pollution is the radioactive contamination that results from the detonation of nuclear devices, the waste products of nuclear power plants, and from conventional weapons using depleted uranium. The main aim of this study is the development of novel sensing technologies for detection of radiation pollution using microorganisms, such as bacteria. The most common bacteria, Escherichia coli (E. coli for short) was selected for this study. Several experimental techniques, such as fluorescence microscopy, fluorescence spectroscopy, and light scattering (OD600) were utilized for counting living bacteria. The results obtained for freshly prepared bacteria samples were compared to those after exposure to Gamma-radiation (Co-57 source with the dose equivalent to 2000 μSv/h was used) for a different times (from 1 to 60 hours). The results showed that the ratio of living/dead bacteria goes down exponentially with the accumulated dose of radiation. In addition, the ability of E. coli to attenuate Gamma-Radiation was researched, the attenuation factor was found to be of 0.2 cm-1

 

Keywords: Gamma radiation detection, bacteria, E. coli, fluorescence microscopy, UV-vis absorption and fluorescence spectroscopy

 

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