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Vol. 152, No. 5, May 2013, pp. 180-185




A Glucose Sensor Based on Glucose Oxidase Immobilized by Electrospinning Nanofibrous Polymer Membranes
Modified with Carbon Nanotubes
1 You Wang, 1Hui Xu, 2 Zhengang Wang, *1 Ruifen Hu, 3 Zhiyuan Luo, 2 Zhikang Xu, 1 Guang Li

1 National Laboratory of Industrial Control Technology, Institute of Cyber-systems and Control, Zhejiang University, Hangzhou 310027, China

2 Institute of Polymer Science, Key Laboratory of Macromolecular Synthesis and Functionalization, Zhejiang University, Hangzhou 310027, China

3 Computer Learning Research Centre, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK

Tel.: +86-57187952268, fax: +86-57187952279

E-mail: 0011377@zju.edu.cn


Received: 30 March 2013   /Accepted: 15 May 2013   /Published: 27 May 2013

Digital Sensors and Sensor Sysstems


Abstract: A glucose biosensor based on glucose oxidase immobilized by electrospinning nanofibrous membranes has been developed. Nanofibrous membranes were electrospun from the solution of poly(acrylonitrile-co-acrylic acid) containing carbon nanotubes suspension and directly deposited on Pt electrodes for immobilizing glucose oxidase. The morphologies and structure of the nanofibrous membranes with or without carbon nanotubes were characterized by scanning electron microscopy. The fabrication parameters of nanofibers were optimized such as thickness of the nanofibrous membranes and mass ration of carbon nanotubes. The biosensor showed the relationship with a concentration range of 0.110 mM and response time was 60 s. The sensitivity of carbon nanotubes modified biosensors was two times larger than which of no carbon nanotubes modified ones. The pH effect, interference and lifetime of biosensors were discussed.


Keywords: Electrospinning, Glucose biosensor, Enzyme immobilization, Carbon nanotubes


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