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Vol. 79, Issue 5, May 2007, pp. 1192-1201

 

Bullet

 

Lactate Biosensor Based on Cellulose Acetate Membrane Bound Lactate Oxidase

 

Sumana and C. S. Pundirb

aShriram Institute for Industrial Research, 19 University Road, Delhi- 110007, India

bDepartment of Biochemistry and Genetics, M.D. University, Rohtak-124001, India

Tel: + 91-01262-272012,

E-mail: sumanvs@yahoomail.com, Pundircs@rediffmail.com

 

 

Received: 2 April 2007   /Accepted: 11 May 2007   /Published: 31 May 2007

 

Abstract: Lactate biosensor was fabricated by immobilizing lactate oxidase in cellulose acetate membrane and by mounting over the sensing part of Pt electrode (working) and connected to Ag/AgCl electrode (reference) along with auxillary electrode through potentiostat. The enzyme electrode was anodically polarized at +400 mV to generate electrons from H2O2, which was formed from oxidation of serum lactate by immobilized lactate oxidase. The minimum detection limit of the electrode was 0.1mmoles/L and sensitivity of the sensor was 0.008 mA/mM/L lactate. Assay coefficients of variation were < 2% .A good correlation (r=0.99) was found between lactate values obtained by colorimetric method and lactate biosensor. The self-life of the biosensor was 18 days at 4șC and enzyme electrode can be re-used 150 times without any significant loss in enzyme activity.

 

Keywords: Lactate biosensor, Lactate oxidase, CA membrane, Amperometric biosensor

 

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