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Vol. 161, Issue 12, December 2013, pp. 452-459




An Amperometric Immunosensor Based on Graphene-Multi-Walled Carbon Nanotubes-Chitosan Composite Film
for Chlorpyrifos Detection
Yemin Guo, Yaoyao CAO, * Xia SUN, * Xiangyou WANG

School of Agriculture and Food Engineering, Shandong University of Technology, No. 12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China
Tel.:+86-533-2786558, fax: +86-533-2786558
E-mail: sunxia2151@sina.com, wxy@sdut.edu.cn


Received: 1 October 2013   /Accepted: 22 November 2013   /Published: 30 December 2013

Digital Sensors and Sensor Sysstems


Abstract: In this paper, we have designed a kind of immunosensor for the detection of chlorpyrifos. Graphene and multi-walled carbon nanotubes could be dissoved with chitosan, thus the graphene-multi-walled carbon nanotubes-chitosan (GR-MWCNTs-CHIT) nanocomposite film was obtained. Gold nanoparticles can be bound through Au-N bond with chitosan. Then the modified GCE was immersed in the anti-chlorpyrifos antibody solution. Finally, bovine serum albumin (BSA) was employed to block the possible remaining active sites avoiding any nonspecific adsorption. The fabrication procedure of the immunosensor was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), respectively. Under optimized conditions, the current response was proportional to the concentration of chlorpyrifos which ranged from 0.1 to 1.0 ? 105 ng/mL with a detection limit of 0.057 ng/mL. The proposed chlorpyrifos immnuosensor exhibited high specificity, reproducibility, and stability performance, which may open a new door for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.


Keywords: Graphene, Multi-walled carbon nanotubes, Chitosan, Gold nanoparticles, Chlorpyrifos, Immunosensor.


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