bullet Sensors & Transducers Journal

    (ISSN 1726- 5479)

 

 

25 Top Downloaded Articles

Editorial Calendar 2007

Submit an Article

Editorial Board

Current Issue

Sensors & Transducers Cover

 

Sensors & Transducers Journal 2006

2000-2002 S&T e-Digest Contents

2003 S&T e-Digest Contents

2004 S&T e-Digest Contents

2005 S&T e-Digest Contents

 

  Best Articles 2006

   

 

 

Vol. 82, Issue 8, August 2007, pp. 1458-1469

 

Bullet

 

Lactase from Clarias Gariepinus and its Application in Development of Lactose Sensor

 

Sandeep K. SHARMA a,, Neeta SEHGAL b and Ashok KUMAR a

a Institute of Genomics and Integrative Biology, Mall Road, Delhi, India, 110 007

b Department of Zoology, Delhi University, Delhi, India, 110 00

 Phone : +91 11 27666156, E-mail: ashokigib@rediffmail.com

 

 

Received: 8 March 2007   /Accepted: 20 August 2007   /Published: 27 August 2007

 

Abstract: A natural source of lactase enzyme was reported for the first time from an omnivorous cat fish, Clarias gariepinus. The lactase enzyme has been partially purified from catfish intestine using FPLC. The enzyme had specific activity 6.75 units mg-1 with an overall recovery of 80%. The enzyme was characterized and kinetic properties such as Km(4.8mM),Vmax (11.66 μmoles min-1mg-1), optimum pH(7.2 7.5) and thermal inactivation have been studied. The enzyme was stable on storage at 4 oC with only 5% loss in enzyme activity in 120 days. The energy of activation for thermal inactivation of catfish lactase was 23 K cal mol-1. The partially purified enzyme was used for the fabrication of lactose biosensor.

 

Keywords: Catfish lactase, Clarias gariepinus, Lactose, Lactose intolerance

 

Acrobat reader logo Click <here> or title of paper to download the full pages article (587 Kb)

 

 

 

 


1999 - 2007 Copyright , International Frequency Sensor Association (IFSA). All Rights Reserved.


Home - News - Links - Archives - Tools - Standardization - Patents - Marketplace - Projects - Wish List - Subscribe - Search - e-Shop - Membership

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Sensor Jobs - Site Map