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Vol. 191, Issue 8, August 2015, pp. 46-51




Conductivity of Pedot-Pss with Gold and Silver Nanocomposites Modified Gold Electrodes
for Ganoderma Boninense DNA Detection

1 Sabo Wada Dutse, 2 Nor Azah Yusof, 3 Haslina Ahmad

1 Department of Science Laboratory Technology, Hussaini Adamu Federal Polytechnic, P.M.B 5004, Kazaure, Nigeria
2, 3 Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia
1 Tel.: +2348035860235

E-mail: swdutse@yahoo.com, sabo.wada@hafedpoly.edu.ng


Received: 6 July 2015 /Accepted: 10 August 2015 /Published: 31 August 2015

Digital Sensors and Sensor Sysstems


Abstract: The conductivity of a designed electrochemical DNA biosensor was improved using gold and or silver nanoparticles. A gold electrode modified with a conductive nanocomposite of poly(3,4-ethylene dioxythiophen)–poly (styrenesulfonate) (Pedot-Pss) and gold or silver nano particles enhanced the conductivity of the electrode surface area. Bare and modified gold electrode surfaces were characterized using cyclic voltammetry (CV) technique in ethylenediaminetetraacetic acid (TE) supporting electrolyte. Immobilization of a 20-mer DNA probe was achieved by covalent attachment of the amine group of the capture probe to a carboxylic group of an activated 3,3’-dithiodipropionic acid layer using EDC/NHSS for Hybridization. The effect of hybridization temperature and time was optimized and the sensor demonstrated specific detection for the target concentration ranged between 1.0´10-15 M to 1.0´10-9 M with a detection limit of 9.70´10-19 M. Control experiments verified the specificity of the biosensor in the presence of mismatched DNA sequence. The DNA hybridization was monitored using a new ruthenium complex [Ru(dppz)2(qtpy)Cl2; dppz = dipyrido [3,2–a:2’,3’-c] phenazine; qtpy=2,2’,-4,4”.4’4”’-quarterpyridyl redox indicator.


Keywords: Biosensor, Pedot-Pss, DNA, Nanocomposite, Ruthenium complex.


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