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Vol. 191, Issue 8, August 2015, pp. 52-56




A Method for the Determination of Bi-substrate Kinetic Coefficients:
the Example of the
b-D-glucose- NAD-GDH Enzymatic Reaction

1 Jean BERTHIER, 1 Myriam Cubizolles, 1 Patricia LAURENT, 2 Vincent POHER, 2 Patrick POUTEAU, 1 Marie-Line COSNIER

1 University Grenoble Alpes, F-38000, Grenoble, Department of Biotechnology, CEA, LETI, MINATEC, 38054 Grenoble, France
2 Avalun, 17 avenue des Martyrs, 38054 Grenoble, France
1 Tel.: 0033 438783551

1 E-mail: jean.berthier@cea.fr


Received: 24 June 2015 /Accepted: 30 July 2015 /Published: 31 August 2015

Digital Sensors and Sensor Sysstems


Abstract: Colorimetric detection of glucose in sample liquids such as human plasma is made by using enzymatic reactions. Either glucose oxidase (GOX) or glucose dehydrogenase (GDH) can be used to convert glucose. In the multi reactional scheme, the first enzymatic reaction is determinant. We focused here on the study of the enzyme GDH together with the enzymatic cofactor NAD (nicotinamide adenine dinucleotide). This reaction falls in the category of ternary enzymatic reactions. Such reactions depend on four parameters. A method to determine these four parameters is presented in this work, based on a comparison between a series of experiments and the theory. The best values of the parameters are indicated.


Keywords: Ternary enzymatic reaction, Michaelis-Menten kinetics, Bi-substrate kinetics, Glucose.


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