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Vol. 28, Special Issue, April-May 2018, pp. 32-37

 

Bullet

 

Surface Functionalization of Magnetic Nanoparticles for the Enrichment
and Detection of Albumin in Urine
 

1 Jean Ashley S. TALAL, 2 Mary Kathleen D. PRINCIPE and 1, 2, 3, * Dharmatov Rahula B. ALBANO

1 University of Santo Tomas Graduate School, Espaňa Blvd., Manila 1015, Philippines
2 University of Santo Tomas Department of Chemistry, Espaňa Blvd., Manila 1015, Philippines
3 University of Santo Tomas Research Center for the Natural and Applied Sciences, Espaňa Blvd., Manila 1015, Philippines
1 Tel.: +63 2 4061611 loc. 4045, fax: +63 2 7314031

E-mail: dbalbano@ust.edu.ph

 

Received: 28 March 2018 /Accepted: 28 May 2018 /Published: 31 May 2018

Digital Sensors and Sensor Sysstems

 

Abstract: In recent years, magnetic nanoparticles (MNPS) and their use in biomedical and biotechnological applications have received a lot of attention. In this study, the surface of the iron oxide magnetic nanoparticles was functionalized to achieve high affinity and sensitivity in targeting a specific molecule. The immobilization of avidin on the functionalized MNPs was done in preparation for the coupling of biotinylated aptamers to target human serum albumin for the detection of microalbuminuria. The structural information of functionalized magnetic nanoparticles was characterized using transition electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). The ultra-high performance liquid chromatography (UPLC) was used to detect the trace albumin in spiked buffer samples. It shows that for 0.01 ppm HSA concentration, an uptake of 55.03 1.15 % and an LOD of 1 pbb are observed.

 

Keywords: Iron (II, III) oxide nanomagnetic particles, Strepavidin, Immobilization, Nanoparticle functionalization, Human serum albumin.

 

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