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Vol. 28, Special Issue, April-May 2018, pp. 38-42

 

Bullet

 

Bacteriophage-modified Graphene Oxide Screen-printed Electrodes for the Impedimetric Biosensing
of Salmonella Enterica Serovar Typhimurium
 

1, * Pauline Ann QUITON, 3 Beatrice Marie CARREON, 1, 2, 4 Donna May DELA CRUZ-PAPA and 1, 2, 3 Jose BERGANTIN, Jr.

1 The Graduate School
2 Research Center for the Natural and Applied Sciences
3 Department of Chemistry
4 Department of Biology, College of Science, University of Santo Tomas, Espaňa Blvd., Manila 1015, Philippines
E-mail: pamquiton@gmail.com

 

Received: 28 March 2018 /Accepted: 28 May 2018 /Published: 31 May 2018

Digital Sensors and Sensor Sysstems

 

Abstract: In this study, an electrochemical impedance biosensor was developed using an isolated bacteriophage as a bioreceptor to detect Salmonella enterica serovar Typhimurium. The bacteriophage was immobilized on graphene oxide- modified screen-printed electrode (GO-SPE) using 1-(3-dimethylaminopropyl)- ethylcarbodiimide hydrochloride (EDC) as a crosslinker. The modified GO-SPEs were characterized using Field Emission Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy to confirm the successful immobilization of the bacteriophage on the electrode surface. Electrochemical Impedance Spectroscopy (EIS) was used to monitor variations in the charge transfer resistance (Rct) values at different immobilization and measurement conditions. The fabricated GO-SPE/Phage biosensor displayed quantitative response for S. Typhimurium over a broad range of concentrations (10-1 108 cfu/mL) and an increasing linear response was observed (R2=0.9842). The optimized incubation time and response time were 30 min and 4 min, respectively. Selectivity tests showed that the biosensor is selective towards S. Typhimurium over other bacteria, such as Escherichia coli and Staphylococcus aureus.

 

Keywords: Biosensors, Bacteria detection, Bacteriophage, Electrochemical Impedance Spectroscopy, Salmonella, Screen-printed electrode.

 

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