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Vol. 101, Issue 2, February 2009, pp.112-122

 

 

Bullet

 

Development of an Optical Urea Biosensor Using Polypyrrole-polyvinyl Sulphonate Film

 

H. J. KHARAT, K. DATTA, P. GHOSH, *MAHENDRA D. SHIRSAT

Optoelectronics and Sensor Research Laboratory, Department of Physics,
Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431 004, Maharashtra, India

*Tel.: +91- 0240 - 2400431 437, fax: +91- 0240 2400291

*E-mail: mdshirsat_bamu@yahoo.co.in

 

 

Received: 15 December 2008   /Accepted: 20 February 2009   /Published: 28 February 2009

 

Abstract: Development of an intensity modulated fiber optic PPy-PVS biosensor for the detection of urea has been presented. The sensor design is based on the modified cladding technique. Polypyrrole (PPy) film doped with polyvinyl sulfonate (PVS) was synthesized (by in-situ chemical polymerization at room temperature) with optimized process parameters. The PPy-PVS film provides good porous matrix for the immobilization of enzyme-urease on the sensing probe by cross linking via glutaraldehyde. The characterization of the urea biosensor has been carried out with an indigenously developed sensing chamber and optical fiber test bench. This biosensor showed almost stable and linear response to urea in the range 1-100 mM up to 24 days. It shows very good selectivity and repeatability.

 

Keywords: Optical fiber, modified cladding, Polypyrrole, Urease, Urea biosensor

 

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