bullet Sensors & Transducers Journal

    (ISSN 1726- 5479)


2008 e-Impact Factor

25 Top Downloaded Articles

Journal Subscription 2010

Editorial Calendar 2010

Submit an Article

Editorial Board

Current Issue

Sensors & Transducers journal's cover

Sensors & Transducers Journal 2008

Sensors & Transducers Journal 2007

2000-2002 S&T e-Digest Contents

2003 S&T e-Digest Contents

2004 S&T e-Digest Contents

2005 S&T e-Digest Contents

2006 S&T e-Digest Contents


Best Articles 2008




Vol. 113, Issue 2, February 2010, pp. 115-126




Development of Piezoelectric DNA-Based Biosensor for Direct Detection of Mycobacterium Tuberculosis in Clinical Specimens



1Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand

Tel.: +66 2260-2122 ext 4605, fax: +66 2260-0125.

E-mails: tkaewphinit@yahoo.com, kosum@swu.ac.th

2Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand

E-mail: titi41@yahoo.com

3Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Prannok, Bangkok, 10700, Thailand

E-mail: mtcpm@mahidol.ac.th



Received: 26 December 2009   /Accepted: 19 February 2010   /Published: 26 February 2010


Abstract: This study was focused on establishment of piezoelectric biosensor for direct detection of Mycobacterium tuberculosis (MTB) in clinical specimens. The quartz crystal immobilized via 3-mercaptopropionic acid (MPA)/avidin/DNA biotinylated probe on gold surface and hybridization of the DNA target to DNA biotinylated probe. The optimal concentration of MPA, avidin and 5-biotinylated DNA probe for immobilization of specific DNA probe on gold surface were 15 mM, 0.1 mg/ml and 1.5 μM, respectively. The detection of genomic DNA digestion in the range from 0.5 to 30 μg/ml. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex (MAC) and other microorganism. This target DNA preparation without amplification will reduce time consuming, costs, and the tedious step of amplification. This study can be extended to develop the new method which is high sensitivity, specificity, cheap, easy to use, and rapid for detection of MTB in many fields.


Keywords: Mycobacterium tuberculosis, Piezoelectric biosensor, Hybridization, Digestion genomic DNA


Acrobat reader logo Click <here> or title of paper to download the full pages article (893 Kb)





1999 - 2010 Copyright , International Frequency Sensor Association (IFSA). All Rights Reserved.

Home - News - Links - Archives - Tools - Standardization - Patents - Marketplace - Projects - Wish List - Subscribe - Search - Membership - Submit Press Release

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Sensor Jobs - e-Shop - Site Map