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Vol. 113, Issue 2, February 2010, pp. 127-139

 

Bullet

 

An Electrochemical Oxalate Biosensor Based on CA Membrane Bound Sorghum Oxalate Oxidase

 

R. CHAUDHARY and *C. S. PUNDIR

Biochemistry Research Lab, Department of Biochemistry M. D. University,

Rohtak-124001 Haryana, India

* Fax: (91)1262-295480

E-mail: pundircs@rediffmail.com

 

 

Received: 31 January 2010   /Accepted: 19 February 2010   /Published: 26 February 2010

 

Abstract: A method is described for construction of an electrochemical oxalate biosensor using CA membrane bound sorghum leaf oxalate oxidase. The biosensor showed optimum response within 2 min at 0.65V, pH 5.5 and 35 C. The biosensor was employed for amperometric determination of oxalate in urine was. The minimum detection limit of the method was 0.02mmoles/L of urine. Analytical recovery of added oxalate was 95.9 %. Within and between assay coefficients of variation were <3.1% and <6.5 % respectively. A good correlation (r=0.982) was found between oxalate values obtained by a modified Sigma kit method and the present biosensor. The enzyme electrode lost 50 % of its initial activity after its 100 uses over a period of 30 days, when stored at 4 C. The electrode has the advantage over barley enzyme electrode, as it is free from interference by Cl and NO3 normally found in biological fluids.

 

Keywords: Oxalate, Oxalate oxidase, Oxalate biosensor, Urine, Sorghum leaf, CA membrane

 

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