Vol. 113, Issue 2, February 2010, pp. 140-149

Detection of a BSA-Biotin-Conjugate by a Novel Immunosensor

^a#Lok Hang MAK, ^bMeinhard KNOLL, ^cNico DANKBAR, ^aTanja FISBECK,

^a,*Andreas GORSCHLÜTER

^aPhysikalisches Institut, Universität Münster, Wilhelm Klemm-Str.10, D-48149 Münster, Germany

^bInstitut für Physikalische Chemie, Universität Münster, Corrensstr. 30, D-48149 Münster, Germany

^cDepartment of Tumor Biology, University Medical Center Hamburg-Eppendorf

#Current address: Imperial College London, Exhibition Road, London, SW7 2AZ, UK

*Tel.: +49-0251-8333610, fax: +49-0251-8336351

E-mail: a.gorschlueter@uni-muenster.de

Received: 16 November 2010   /Accepted: 19 February 2010   /Published: 26 February 2010

Abstract: We describe a new kind of biosensor for the electro-magnetic sensing of analytes such as antibodies. In the present study the molecules bovine serum albumin (BSA) and the corresponding antibody (Anti-BSA) as well as biotin and NeutrAvidin are used as model systems. The analyte molecules are labelled with superparamagnetic microbeads and detected by sensor transducers with biofunctionalised microelectrodes. It is shown that a carboxyl-modified dextran (CMD) layer is well suited as an immobilization matrix for ligands on the microelectrode surface. The CMD layer offers a suitable and stable surface for binding of the antibodies and also suppresses unspecific binding of protein/antibody coated microbeads to the electroactive platinum surface.

Keywords: Microbeads, Superparamagnetic, Microelectrode, Intermolecular forces, Sandwich assay

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