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Vol. 119, Issue 8, August 2010, pp.129-141




Amperometric Glucose Biosensor based on Immobilization of Glucose Oxidase in Polyethylenemine
and Poly (carbamolylsuphonate) Polymer Matrix


aU. B. Trivedi, aD. Lakshminarayana, bI. L. Kothari, cN. G. Patel, dH. N. Kapse, aP. B. Patel, e*C. J. Panchal

aDepartment of Electronics, bDepartment of Bio-Sciences, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India

cDepartment of Chemistry and Physics, University of North Florida, Jacksonville, FL, USA

dInstitute of Science and Technology for Advanced Studies and Research (ISTAR), Vallabh Vidyanagar-388120, Gujarat, India

eApplied Physics Department, Faculty of Technology and Engineering, M. S. University of Baroda, Vadodara-390001, Gujarat, India

*Tel.: +91 9825094761, fax: +91 265 2423898

E-mail: cjpanchal_msu@yahoo.com



Received: 19 June 2010   /Accepted: 17 August 2010   /Published: 31 August 2010


Abstract: A low cost, easy to fabricate, simple to operate, portable and disposable biosensor based upon the enzyme glucose oxidase (GOD) has been developed. A polymer matrix of Polyethylenemine (PEI) and Poly Carbamoylsulphonate (PCS) hydrogel has been used for the immobilization of GOD on a Platinum (Pt) tip of a screen printed graphite electrode. A multi channel potentiostat has been utilized in the electrode configuration for the amperometric measurement of glucose. The developed Pt/PCS+PEI/GOD glucose biosensor system could offer a reliable method of glucose determination in standard stock solutions. The designed glucose bio-sensor system has been duly characterized and the results obtained are presented. The biosensor has been found to show a sensitivity of 0.72 0.15 nA/M. Also, the specificity of the enzyme electrode and the storage stability of the GOD based biosensor system have been studied. The sensor system has also been tested with the real samples of fruit juice, soft drink and human blood. The biosensor system has been found to possess a potential for food analysis and clinical utility.


Keywords: Biosensor, Amperometry, Glucose, Glucose oxidase, Polymer matrix


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