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Vol. 119, Issue 8, August 2010, pp.142-150




Development of Bio-analyzer for the Determination of Urinary Chloride


1R. Vasumathi , 2*P. Neelamegam

1Department of Physics, A.V.V .M Sri Pushpam College,
Poondi, Thanjavur, Tamilnadu India, 613503

2*Department of Electronics and Instrumentation Engineering, SASTRA University,

Thanjavur, Tamilnadu, India

*E-mail: neelkeer@hotmail.com



Received: 5 March 2010   /Accepted: 17 August 2010   /Published: 31 August 2010


Abstract: A high performance Microcontroller P89C668 based Biomedical Analyzer to measure the Urinary Chloride Concentration is designed and developed. The implemented system incorporates light source, Photodiode GASPG1104, Sample holder, LCD for displaying patient test results and key board for executing functions. The details of the interfacing circuit and the software to compute the concentration of Chloride ion is explained in this paper. To determine the run to run precision of the implemented system, human control serum AccutrolTM Normal (Sigma) is analyzed and the Chloride concentration is found to be 10 4 mmol/l (mean SD, n=5), which closes to the certified value. Good recovery results are obtained with the range of 98 % to 99 %. The results obtained using the designed instrument is in agreement with those obtained by the clinical analyzer. The sensitivity and linearity of the microcontroller based instrument are high enough to determine the concentration of Chloride ion without any significant interference.


Keywords: Chloride ion, Colorimetry, Bio-analyzer, Urine sample


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