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Vol. 67, Issue 5, May 2006, pp. 561-567

 

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Preparation of Oxygen Meter Based Biosensor for Determination of Triglyceride in Serum

 

M. BHAMBI, MINAKSHI and C.S. PUNDIR*

Biochemistry Research Laboratory, Deptt of Bio-Science, M D University,

Rohtak 124001 Haryana, India

* Author for correspondence Fax: 91 1262 274076; E-mail: pundircs@yahoo.com

 

 

Received:  11 March 2006   Accepted: 24 May 2006   Published: 29 May 2006

 

Abstract. A method is described for preparation of a dissolved oxygen meter (make Aqualytic, Germany) based triglyceride biosensor employing a polyvinyl chloride (PVC) membrane bound lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase The biosensor measures dissolved O2 utilized in the oxidation of triglyceride (TG) by membrane bound lipase, glycerol kinase (GK) and  glycerol-3-phosphate oxidase (GPO), which is directly proportional to (TG) concentration. The biosensor showed optimum response within 10-15 sec at pH 7.5 and 39.5 șC. A linear relationship was obtained between the (TG) concentration from 5mM to 20mM and oxygen consumed (mg/L). The biosensor was employed for determination of triglyceride in serum. The within and between batch coefficient of variation (CV) were < 2.18 % and < 1.7% respectively. The minimum detection limit of the biosensor was 0.35 mM. A study of interference revealed that ascorbic acid, cholesterol and bilirubin  caused 13%, 15%, and 12% interference, respectively.The biosensor is portable and can be used outside the laboratory.

 

Keywords: triglycerides, lipase, GK, GPO, co-immobilization, enzyme electrode , dissolved oxygen meter, polyvinyl chloride membrane, serum

 

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