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Vol. 122, Issue 11, November 2010, pp.85-101



Catechol Biosensor Based on Gold Nanoparticle Modified Tetrabutylammoniumtetrafluoroborate
Doped Polythiophene Films


a*Suman SINGH, aS. Praveen KUMAR, bD. V. S. JAIN, a*M. L. SINGLA

a Central Scientific Instruments Organization (CSIR), Sector 30-C, Chandigarh, India

b Panjab University, Chandigarh, India

*Email: sumansingh01@gmail.com



Received: 17 August 2010   /Accepted: 18 November 2010   /Published: 30 November 2010


Abstract: Tetra butyl ammonium tetra fluoroborate (TBATFB) doped polythiophene films have been polymerized galvano-statically which were then modified with soium citrate capped gold nanoparticles (AuNPs) using dip coating method. Catechol biosensor was fabricated using laccase enzyme. ITO/Pth and ITO/Pth/AuNPs/Lac bioelectrodes were characterized using scanning electron microscopy (SEM), UV-Visible spectroscopy and electrochemical techniques. Absorbance for ITO/Pth/AuNPs/Lac bioelectrodes was monitored at 410 nm as a function of catechol concentration and pH. Cyclic voltammetric studies of ITO/Pth electrodes showed quasi-reversible behavior when recorded in sodium acetate buffer, which later turned into reversible behavior on modification with gold nanoparticles. ITO/Pth/AuNPs/Lac bioelectrodes showed maximum oxidation potential for enzymatic reaction of catechol at -0.2 V, with linear range upto 0.8 mM and regression coefficient (R2) of 0.988. Sensitivity and Km values were found to be 3.7 x 10-5 mA/mM and 0.22 mM respectively, with response time of about 6-8 s.


Keywords: Polythiophene, Gold nanoparticles (AuNPs), Catechol, Laccase.


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