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Vol. 126, Issue 3, March 2011, pp.11-18

 

Bullet

 

Fabrication of a Highly-sensitive Acetylcholine Sensor Based on AChOx Immobilized Smart-chips

 
M. M. RAHMAN

Venture Business Laboratory, Department of Electrical and Electronic Engineering, Toyohashi University of Technology,

1-1 Hibarigaoka, Tempeku-cho, Toyohashi, Aichi 441-8580, Japan

Tel.: +81-532-44-6974

E-mail: mmrahmanh@gmail.com

 

 

Received: 12 January 2011   /Accepted: 17 March 2011   /Published: 29 March 2011

 

Abstract: Acetylcholine (ACh) sensor based on acetylcholine oxidase (AChOx) on EDC activated thioglycolic acid self-assembled monolayer (TGA-SAM) using smart-chip has been developed. The simple cyclic voltammetry (CV, at 0.1 V/s) technique is performed in total investigation, where 0.5M K3Fe(CN)6 is utilized as a standard mediator in phosphate buffer solution (PBS, 0.1M). The ACh sensor exhibited a lower detection limit (DL, 0.1392 0.005 nM), a wide linear dynamic range (LDR, 1.0 nM to 1.0 mM), good linearity (R=0.9951), and higher sensitivity (7.3543 0.2 μAμM-1cm-2), and required small sample volume (70.0 μL) as well as good stability and reproducibility. The smart-chip system employed a simple and efficient approach to the immobilization of enzymes onto active sensitive surface, which can enhance sensor performances to a large group of bio-molecules for wide range of biomedical applications in health care fields.

 

Keywords: Acetylcholine, Acetylcholine esterase, Cyclic voltammetry, Smart-chip, Mediator

 

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