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Vol. 134, Issue 11, November 2011, pp.177-186

 

Bullet

 

Amperometric Acetylcholinesterase Biosensor Based on Multilayer Multiwall Carbon Nanotubes-chitosan Composite
 

Xia SUN, Chen ZHAI, Xiangyou WANG

School of Agriculture and Food Engineering, Shandong University of Technology,
No.12, Zhangzhou Road, Zibo 255049, Shandong Province, P. R. China

Tel.:+86-533-2786558, fax: +86-533-2786558

E-mail: wxy@sdut.edu.cn

 

 

Received: 21 October 2011   /Accepted: 21 November 2011   /Published: 29 November 2011

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Abstract: A simple method for immobilization of acetylcholinesterase (AChE) onto the glassy carbon electrode (GCE) modified with five layers of multiwall carbon nanotubes (MWNTs)-chitosan (CHIT) composite was proposed, and thus a fast, sensitive and stable amperometric sensor for quantitative determination of pesticides was developed. Five layers of MWNTs-CHIT promoted electron transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus, it improved the detection sensitivity of biosensor. Based on the inhibition of pesticides to the enzymatic activity of AChE, using carbofuran as a model compound, under optimal conditions, the inhibition of carbofuran was proportional to its concentration in two ranges, from 510-4 to 7.5 μg/mL and 7.5 to 20 μg/mL with a detection limit of 110-4 μg/mL. The constructed biosensor showed prominent characteristics and performances such as good precision, acceptable stability, fast response and low detection limit, which provided a new promising tool for pesticide analysis.

 

Keywords: Biosensor, Acetylcholinesterase, Multiwall carbon nanotubes, Chitosan

 

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