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Vol. 134, Issue 11, November 2011, pp.187-201

 

Bullet

 

Fabrication of Biosensors Based on Nanostructured Conducting Polyaniline (NSPANI)
 

*1 Deepshikha SAINI, 2 Ruchika CHAUHAN and 2 Tinku BASU

1 Amity Institute of Biotechnology, Amity University Uttar Pradesh,

Noida, India-201301

2Amity Institute of Nanotechnology, Amity University Uttar Pradesh,

Noida, India-201301, E-mail: dshikha@amity.edu

 

 

Received: 11 August 2011   /Accepted: 21 November 2011   /Published: 29 November 2011

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Abstract: In this study, glucose and hydrogen peroxide (H2O2) biosensors based on nanostructured conducting polyaniline (NSPANI) (synthesized using sodiumdodecyl sulphate (SDS) as structure directing agent) were developed. Because of the large specific surface area, excellent conductivity of NSPANI, horseradish peroxidase (HRP) and glucose oxidase (GOx) could be easily immobilized with high loading and activity. In addition the small dimensions and the high surface-to-volume ratio of the NSCP allow the rapid transmission of electron and enhance current response. The linear dynamic range of optical glucose and H2O2 biosensors is 540 mM for glucose and 150 mM for H2O2, respectively where as the bulk PANI exhibits linearity between 5-20 mM/l. The miniature optical glucose biosensor also exhibits good reproducibility. The storage stability of optical glucose and H2O2 biosensors is two weeks for glucose and five days for H2O2. The high response value of NSPANI based biosensors as compared to bulk PANI based biosensor reflects higher enzymatic affinity of GOx/NSPANI and HRP/NSPANI with glucose and H2O2 due to biocompatibility, active surface area and high electron communication capability of nanobiopolymer film. In conclusion, the NSPANI based biosensors proposed herein have many advantages such as a low response time, high reproducibility, high sensitivity, stable and wide dynamic range.

 

Keywords: Polyaniline nanoparticles, Enzyme biosensors, Glucose oxidase, Horseradish peroxidase, Glucose, Hydrogen peroxide, Spectroelectrochemistry

 

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