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Vol. 13, Special Issue, December 2011, pp.131-141

 

Bullet

 

A Model for Enhanced Chemiluminescence Reactions in Microchambers Combined to an Active Pixel Sensor

 
Jean BERTHIER, Pierre L. JOLY, Florence RIVERA, Patrice CAILLAT

CEA-LETI-Minatec, Department of Biotechnology,
17 avenue des Martyrs, 38954, Grenoble, France

Tel.: +33 4 3878 3551

E-mail: jean.berthier@cea.fr

 

 

Received: 29 June 2011   /Accepted: 16 November 2011   /Published: 28 December 2011

Handbook of Laboaratory Measurements book

 

Abstract: Detection of hybridized targets - like DNA strands -in microsystems for biotechnology is usually done by fluorescence. However, recent investigations have shown that chemiluminescence could be a more effective and sensitive tool for detection under the condition that dispersion of light due to diffusion is not too important. In this study, a model for an enhanced chemiluminescence reaction using luminols is proposed and the resulting light emission and absorption by the CMOS APS (active pixel sensor) is predicted.

 

Keywords: Luminol, Diffusion, Michaelis-Menten model, APS (Active pixel sensor)

 

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