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Vol. 13, Special Issue, December 2011, pp.142-149

 

Bullet

 

Antibody Immobilization on Conductive Polymer Coated Nonwoven Fibers for Biosensors

 

1 Shannon K. MCGRAW, 1 Michael J. ANDERSON, 1 Evangelyn C. ALOCILJA, 2 Patrick J. MAREK, 3 Kris J. SENECAL, 2 Andre G. SENECAL

1 Biosystems and Agricultural Engineering, Michigan State University, 115 Farrall Hall, East Lansing, MI, 48824, USA

2 Food Safety and Defense Team, US Army Natick Soldier Research Development and Engineering Command (NSRDEC),

Kansas St., Natick, MA, 01760, USA

3 Macromolecular Sciences and Engineering Team, US Army NSRDEC, Kansas St., Natick, MA, 01760, USA

E-mail: alocilja@msu.edu

 

 

Received: 29 June 2011   /Accepted: 16 November 2011   /Published: 28 December 2011

 

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Abstract: This work is being performed to develop rapid and novel electrochemical biosensors for foodborne pathogen detection. This research focuses on electrotextile platforms to perform both capture and sensing functions in a single component. The biosensor uses nonwoven fiber membranes coated with conductive polymer and functionalized with antibodies for biological capture. This study examines three methods for antibody immobilization: passive adsorption, glutaraldehyde cross-linking, and EDC/Sulfo-NHS cross-linking. Antibodies are immobilized onto the conductive fiber surfaces for the specific capture of a target pathogen. The immobilization and capture capabilities of each method are analyzed through the use of two different fluorescent reporters: FITC and PicoGreen DNA stain. Fluorescence is measured using a fluorescent plate reader and then imaged using a fluorescent microscope. The effect of a blocking agent on specificity is also evaluated. It is found that glutaraldehyde with blocking is the best immobilization method with PicoGreen being the best fluorescent reporter.

 

Keywords: Electrochemical biosensor, Electrotextile, Antibody immobilization, Fluorescence, Immunosensor

 

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