Vol. 14-1, Special Issue, March 2012, pp.185-196

Abstract: Ion channels are transmembrane proteins responsible of cell signaling and a large part of pharmaceutical compounds are interacting with them. In-vitro testing of ion channels is a promising technique for high throughput screening (HTS) in drug discovery and personalized medicine. Automated tests of single ion channels embedded in artificial bilayer lipid membranes (BLM) is gaining attention over patch clamp technique due to its characteristic of performing parallel tests on selected sets of channels or multiple pharmaceutical targets. However, BLM arrays formation is a critical process based on manual and time-consuming techniques. In this paper, an automatic liquid dispensing system for BLM formation monitored in real-time by using low-noise current amplifiers is presented. As proof of this approach, concurrent formation of BLMs is shown. Additionally, single ion channel recordings on an automatically formed BLM is presented and discussed.

Keywords: Bilayer lipid membrane, automatic dispensing system, ion channels, high throughput screening

Click <here> or title of paper to download the full pages article (1.72 Mb)

Read more about Chemical Sensors and Biosensors

1999 - 2012 Copyright ©, International Frequency Sensor Association (IFSA). All Rights Reserved.

Home - News - Links - Archives - Tools - Voltage-to-Frequency Converters - Standardization - Patents - Marketplace - Projects - Wish List - e-Shop - Sensor Jobs - Membership - Videos - Publishing - Site Map - Subscribe - Search

 Members Area -Sensors Portal -Training Courses - S&T Digest - For advertisers - Bookstore - Forums - Polls - Submit Press Release - Submit White Paper - Testimonies - Twitter - Facebook - LinkedIn