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Vol. 14-1, Special Issue, March 2012, pp.266-275

 

Bullet

 

Cell-Culture Real Time Monitoring Based on Bio-Impedance Measurements

 

2 Paula DAZA, 1 Daniel CAÑETE, 1* Alberto OLMO, 1 Juan A. GARCÍA and 1*Alberto YÚFERA

*  Instituto de Microelectrónica de Sevilla (IMSE), Centro Nacional de Microelectrónica (CNM-CSIC)

Universidad de Sevilla, Av. Américo Vespucio s/n. 41092. Sevilla, Spain

1 Dto. Tecnología Electrónica, Universidad de Sevilla, Av. Reina Mercedes, s/n. 41010, Sevilla, Spain

2 Dpt. Cell Biology, Biology Faculty, Seville University,

Av. Reina Mercedes s/n, 41012, Sevilla, Spain

E-mail: yufera@imse-cnm.csic.es, dani@zariweyo.es, aolmo@dte.us.es, hardcorejuin@gmail.com, pdaza@us.es

 

Received: 15 November 2011   /Accepted: 20 December 2011   /Published: 12 March 2012

Digital Sensors and Sensor Sysstems

 

Abstract: This paper proposes the application of a cell-microelectrode model in cell biometry experiments, using the cell-electrode area overlap as its main parameter. The model can be applied to cell size identification and cell count, and further extended to study cell growth and dosimetry protocols. Experiments have been conducted in AA8 cell line, obtaining promising results.

 

Keywords: Microelectrode, ECIS, Bio-impedance, Impedance sensor, Cell culture, Dosimetry

 

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