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Vol. 15, Special Issue, October 2012, pp. 59-70

 

Selected papers from Nanotech Conference & Expo (NanoTech' 2012),
18-21 June 2012, Santa Clara, California, USA

 

Bullet

 

Evaluation of Anchoring Materials for Ultra-Sensitive Biosensors Modified with Au Nanoparticles and Enzymes

 

1 Solomon W. Leung, 2 David Assan and 3 James C. K. Lai

1 Civil and Environmental Engineering Department, School of Engineering and Biomedical Research Institute, Idaho State University, Pocatello, ID 83209, USA

Tel.: 1-208-282-2524, fax: 1-208-283-4538

2 Civil and Environmental Engineering Department, School of Engineering, Idaho State University, Pocatello, ID 83209, USA

3 Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy and Biomedical Research Institute, Idaho State University, Pocatello, ID 83209, USA

E-mail: leunsolo@isu.edu

 

Received: 14 September 2012   /Accepted: 1 October 2012   /Published: 8 October 2012

Digital Sensors and Sensor Sysstems

 

Abstract: In this report, we investigated the performance of ultra high performance sensors fabricated with identical biocomposite materials and procedures, except the anchoring conductive materials. The anchoring materials were glassy carbon, Pt, Au and Ag; the biocomposite layer consisted of polymer/Au nanoparticles/enzyme. The enzymes in the biocomposite layers are specific for the target detecting species which enable the coupling (detection) reactions to occur. The enzymes used in this study were lactate dehydrogenase (LDH), glutamate dehydrogenase (GDH), and hemoglobin. The specific target species for the detection included lactate, NH4+, NO2-, and peroxide. The biosensors developed here were tested with solution concentrations ranged from 10-4 to 10-16 M, however, the lower concentration limit could be orders of magnitude lower. In all, the anchoring materials have drastic effect to the performance of this ultra-high performance biosensor platform; also, nature of the enzymes can alter the stability of these sensors.

 

Keywords: Performance, Anchoring material, Nanoparticle, Sensor, Bioenzymes

 

 

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