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Vol. 144, No. 9, September 2012, pp. 161-181




Recent Advance in Antibody or Hapten Immobilization Protocols of Electrochemical Immunosensor for Detection of Pesticide Residues
Ying ZHU, * Xia SUN, * Xiangyou WANG

School of Agriculture and Food Engineering, Shandong University of Technology,
No.12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China

Tel.:+86-533-2786558, fax: +86-533-2786558

E-mail: sunxia2151@sina.com, wxy@sdut.edu.cn


Received: 12 July 2012   /Accepted: 21 September 2012   /Published: 28 September 2012

Digital Sensors and Sensor Sysstems


Abstract: Electrochemical immunosensors have been used to detect or quantify the specific pesticide based on the binding biomolecules (Ab or hapten) onto the transducer surface to interact with the analyte of target (hapten or Ab), resulting in a detectable signal. In terms of the development of electrochemical immunosensor, the Ab/hapten immobilization onto a transducer or a support matrice is a key step in optimizing the analytical performance, such as response, reproducibility, stability, selectivity and regeneration. This paper presents an overview of electrochemical immunosensors for the detection of pesticides residues and various immobilization protocols of Ab or hapten, such as physical adsorption, covalent coupling, entrapment, oriented immobilization, avidin–biotin affinity reaction, self-assembled monolayer, nanoparticles. Future prospects toward the immobilization protocols for the development of electrochemical immunosensor are discussed.


Keywords: Electrochemical immunosensor, Pesticide residues, Immobilization protocols



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