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Vol. 144, No. 9, September 2012, pp. 192-200




L-Asparaginase Extracted From Capsicum annum L and Development of Asparagine Biosensor for Leukemia

Kuldeep KUMAR and Shefali WALIA

Department of Biotechnology, M.M. Modi College, Patiala-147 001 Punjab, India

Tel.: +91-9876089356, fax: +91-175-2212049

E-mail: kuldeepbio@rediffmail.com



Received: 26 June 2012   /Accepted: 21 September 2012   /Published: 28 September 2012

Digital Sensors and Sensor Sysstems


Abstract: Green chillies (Capsicum annum L.) contain appreciable amount of L-asparaginase enzyme and the present work aims at the development of asparagine biosensor for leukemia. It is a novel and diagnostic plant based biosensor for monitoring asparagine levels acute lymphoblastic leukemia (ALL) samples. Different immobilization techniques and response time studies have been carried out to improve the stability of enzyme by these methods. Phenol Red indicator has been coimmobilized with plant asparaginase and color visualization approach has been optimized for various asparagine ranges. For quantitative analysis, immobilized biocomponent is coupled to Ion Sensing Electrode (ISE) of a potentiometeric transducer. The detection limit of asparagine achieved with immobilization techniques such as gelatin, polyacrylamide gel, agar and calcium alginate beads method is 10-1 10-9M. Also, these techniques have been applied for the detection of asparagine in normal (10-4M) and leukemia blood serum samples (10-2M).


Keywords: Capsicum annum, L-asparaginase, Leukemia, Biosensor, Immobilized



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