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Vol. 146, No. 11, November 2012, pp. 121-132




Immunosensor Based on Gold Nanoparticles-multi-walled Carbon Nanotubes-chitosans Composite and Prussian Blue
for Detection of Chlorpyrifos
Xia SUN, Falan LI, Xiangyou WANG

School of Agriculture and Food Engineering, Shandong University of Technology,

No.12, Zhangzhou Road, Zibo 255049, Shandong Province, P.R. China

Tel.:+86-533-2786558, fax: +86-533-2786558

E-mail: sunxia2151@sina.com, wxy@sdut.edu.cn



Received: 18 July 2012   /Accepted: 23 November 2012   /Published: 30 November 2012

Digital Sensors and Sensor Sysstems


Abstract: In this article, a simple and highly sensitive amperometric immunosensor was developed for the detection of chlorpyrifos. The immunosensor was prepared by sequentially electrodepositing prussian blue (PB), dripping composite membrane of chitosan (CTS), gold nanoparticles (GNPs) and multiwalled carbon nanotubes (MWCNTs) onto glassy carbon electrode (GCE). The electrochemical properties of the modified processes were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), respectively. Multi-walled carbon nanotubes-gold nanoparticles- chitosans composite modified electrode surface was characterized by scanning electron microscopy (SEM). The experimental conditions such as the concentration of antibodies, the incubation temperature, the incubation time and the pH were examined and optimized. Under the optimal conditions, the current response was proportional to the concentration of chlorpyrifos ranging from 0.1 ng/mL to 80 ng/mL and from 90 ng/mL to 200 ng/mL with the detection limit 0.224 ng/mL. The advantages of the immunosensor were exhibited in its wider linear range, better reproducibility, stability, selectivity and regeneration. Using cabbages and Chinese chives as model real samples, acceptable recoveries of 97.1 %-103.4 % was obtained. The proposed method was proven to be a feasible quantitative method for chlorpyrifos analysis, which exhibited high sensitivity, low cost and simplified procedures, for the detection of pesticides residues in food and environment. It may open a new door for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.


Keywords: Electrochemical immunosensor, Prussian blue, Chitosan, Gold nanoparticles, Multiwalled carbon nanotube, Chlorpyrifos



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