Sensors & Transducers Journal

    (ISSN 1726- 5479)

Vol. 82, Issue 8, August 2007, pp. 1458-1469

Lactase from Clarias Gariepinus and its Application in Development of Lactose Sensor

Sandeep K. SHARMA ^a,, Neeta SEHGAL ^b and Ashok KUMAR ^a

^a Institute of Genomics and Integrative Biology, Mall Road, Delhi, India, 110 007

^b Department of Zoology, Delhi University, Delhi, India, 110 00

 Phone : +91 11 27666156, E-mail: ashokigib@rediffmail.com

Received: 8 March 2007   /Accepted: 20 August 2007   /Published: 27 August 2007

Abstract: A natural source of lactase enzyme was reported for the first time from an omnivorous cat fish, Clarias gariepinus. The lactase enzyme has been partially purified from catfish intestine using FPLC. The enzyme had specific activity 6.75 units mg^-1 with an overall recovery of 80%. The enzyme was characterized and kinetic properties such as K_m(4.8mM),V_max (11.66 μmoles min^-1mg^-1), optimum pH(7.2 – 7.5) and thermal inactivation have been studied. The enzyme was stable on storage at 4 ^oC with only 5% loss in enzyme activity in 120 days. The energy of activation for thermal inactivation of catfish lactase was 23 K cal mol^-1. The partially purified enzyme was used for the fabrication of lactose biosensor.

Keywords: Catfish lactase, Clarias gariepinus, Lactose, Lactose intolerance

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