Sensors & Transducers Journal

    (ISSN 1726- 5479)

Vol. 83, Issue 9, September 2007, pp.1555-1563

Cholesterol Biosensor Based on Polyvinyl Formal Membrane Bound Cholesterol Esterase and Oxidase

Ashok Kumar and ^*Suman

Institute of Genomics and Integrative Biology, Mall Road, Delhi-110007, India

^*Shriram Institute for Industrial Research, 19-University Road, Delhi-110007, India

Tel: + 91-01127666156

E-mail: ashokigib@rediffmail.com, sumanvs@yahoo.com

Received: 8 August 2007   /Accepted: 17 September 2007   /Published: 24 September 2007

Abstract: Cholesterol biosensor was fabricated by co-immobilizing cholesterol esterase and cholesterol oxidase in polyvinyl formal membrane and by mounting over the sensing part of oxygen electrode. The immobilized enzymes membrane was characterized by atomic force microscopy. The biosensor showed the linearity for 100-450 mg dl^-1 cholesterol acetate. The minimum detection limit of the cholesterol was 50 mg dl^-1. The effects of temperature, pH and the stability of immobilized enzymes were also studied. A good correlation was found among cholesterol values obtained by colorimetric, Enzo-kit and auto analyzer method. The shelf-life of the biosensor was more than three month at 4ºC and enzyme membrane can be re-used 20 times without any significant loss in enzyme activity.

Keywords: Cholesterol biosensor, Cholesterol esterase, Cholesterol oxidase, PVA membrane

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