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Vol. 211, Issue 4, April 2017, pp. 1-7




Modified Laccase-Gold Nanoparticles-Tetrathiafulvalene-SPCEs Based Biosensor to Determine W(VI) in Water

1 Ana Lorena Alvarado-Gámez, 2 M. Julia Arcos-Martínez

1 School of Chemistry and Centro de Electroquímica y Energía Química, CELEQ, University of Costa Rica, San Pedro de Montes de Oca, 11501-2060, San José, Costa Rica
2 Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain
1 Tel.: 506-25112442, fax: 506-25112446

E-mail: ana.alvaradogamez@ucr.ac.cr


Received: 17 February2017 /Accepted: 31 March 2017 /Published: 30 April 2017

Digital Sensors and Sensor Sysstems


Abstract: It was developed an amperometric biosensor to determine tungsten in water, based on the inhibition of laccase enzyme, by tungsten ions using pyrocathecol as a substrate. The enzyme was immobilized with a proper mixture containing, bovine serum albumin, and glutaraldehyde, for a cross-linking process over screen-printed carbon electrodes, previously modified with tetrathiafulvalen and gold nanoparticles. Optimized experimental conditions are: pyrocatechol in cell 0.040 mM in a phosphate buffer pH 6.5 and applied potential +350 mV. The repeatability and reproducibility, in terms of relative standard deviation values, of de developed biosensor were 3.3 % (n=3), and 2.2 % (n = 5) respectively, and detection limit was 1.8 × 10-7 mol L-1. Additionally it was determined the kinetics of the systems by means of Michaelis-Menten Km apparent constants, calculated using Lineweaver-Burk plots, with and without tungsten. Kinetic study resembles to be competitive inhibition. A recovery study was performed with spiked blanks with a tungsten certified reference standard, traceable to NIST, giving as a result 102.3 ± 6.7 %; tap water samples analyzed presented a mean concentration of 1.75 µM, and recovery of the tungsten certified reference standard on the tap water samples gave 98.8 ±  3.1 %.


Keywords: Biosensor, Cathecol, Gold nanoparticles, Laccase, Screen printed carbon electrodes, Tetrathiafulvalen, Tungsten, Water.


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