Rapid Detection of Pesticide Residues in Vegetables and Fruits via a Hand-Held Instrument

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Vol. 239, Issue 12, December 2019, pp. 17-33

Rapid Detection of Pesticide Residues in Vegetables and Fruits via a Hand-Held Instrument

^1, ^2, ^3, ^* Wenping Zhao, ¹ Hui Zhang, ¹Zhaoqiang Shi, ^1, ^2, ³ Yemin Guo, and ^1, ^2, ³ Xia Sun

¹ School of Agriculture Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
² Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
³ Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zhangdian District, Zibo City, 255000, Shandong Province, P.R. China
¹ Tel.: +86-533-2786558, fax: +86-533-2786558

^* E-mail: 187286528@qq.com

Received: 6 November 2019 /Accepted: 30 November 2019 /Published: 1 December 2019

Abstract: A hand-held instrument for the rapid detection of pesticide residues in vegetables and fruits that was based on acetylcholinesterase (AChE) screen-printed carbon electrode (SPCE) was developed. This device integrated a printable enzyme- based biosensing system and a weak current signal detection circuit. This instrument was coupled with a compact electronic interface for electrochemical detection and real- time wireless data transmission to an internet device. The measurement principle for measuring the pesticide concentration was based on the enzyme inhibition method. The SPCE biosensor was inserted directly into the hand-held instrument to detect pesticides on-site. This biosensor showed a wide linear range for detecting organophosphorus pesticides, extending from 0.05 to 1´10⁵ µg/L, with a detection limit of 0.05 µg/L. The recovery rate for organophosphorus pesticides in vegetables was 89.3 %-103.3 %, and the relative standard deviation was 3.45 %-5.43 %; therefore, the prepared sensor showed good repeatability and stability, and it provided a reliable method for the rapid and real-time detection of organophosphorus pesticide residue.

Keywords: AChE biosensor, Hand-held instrument, Rapid detection, Pesticide residues, Screen-printed carbon electrode.

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