Vol. 137, Issue 2, February 2012, pp. 199-214

Abstract: Amperometric acetylcholinesterase (AChE) biosensors have emerged as a highly sensitive and rapid technique for toxicity analysis in environmental monitoring, food safety and quality control. AChE biosensors have superior properties over the existing techniques such as high-performance liquid chromatography or gas chromatography, because they can provide rapid, sensitive, simple and low-cost on-site detection. This review will highlight the research carried out during the last 5 years on AChE biosensor for determination of organophosphorous and carbamate pesticides in a wide range of samples. In this paper, various immobilization protocols and modified electrode methods used for constructing AChE biosensors are also introduced in detail. Future prospects toward the development of selective, sensitive biosensing systems are discussed.

Keywords: Biosensor, Acetylcholinesterase, Pesticides residue, Immobilization, Modified electrode

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