Vol. 137, Issue 2, February 2012, pp. 215-225

Abstract: Protein coating on solid surface is an essential passivation method to achieve surface biocompatibility for sensing applications. In this paper it was investigated the stability of Bovine Serum Albumine (BSA) coating at different pH values, time and temperature conditions by monitoring the PCR performances on a silicon based lab on chip and by simple contact angle measurement of the coated surface. BSA was deposited on epoxy-silane silicon oxide surface and the coating fully characterized. By means of contact angle values, we found that BSA coating shows surface wettability properties stable under thermal and pH variation, while not treated substrates are very sensible to both pH and temperature stressing. Results here shown indicate that BSA-coated surface is stable in acid, neutral and basic conditions except for pH≥12.

Keywords: PCR, Contact angle, Silane, Surface wettability

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