Rapid Detection of Escherichia coli O157:H7 Using Competitive Exchange of Fluorescent Surrogate Modified Surfaces in Liquid Media

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Vol. 137, Issue 2, February 2012, pp. 254-262

Rapid Detection of Escherichia coli O157:H7 Using Competitive Exchange of Fluorescent Surrogate Modified Surfaces
in Liquid Media

¹ Michael J. ANDERSON, ²Andmorgan R. FISHER, ²Alex LY, ^{1, 3}Evangelyn C. ALOCILJA and ²Clint B. SMITH

¹ Biosystems and Agricultural Engineering, Michigan State University,

115 Farrall Hall, East Lansing, MI, 48824, USA

² US Army Engineer Research and Development Center, Alexandria, Virginia 22315

³ Tel.: 01-517-432-8672, E-mail: alocilja@msu.edu

Received: 24 January 2012 /Accepted: 14 February 2012 /Published: 28 February 2012

Abstract: A novel fluorescent surrogate based immunosensor is presented for use in remote detection of Escherichia coli O157:H7. A total of 3704 confirmed E. coli O157:H7 cases with a hospitalization rate of 46.5 % were reported by the Centers for Disease Control and Prevention in 2008. Rapid detection would aid in both prevention and water supply security. This report describes the successful generation of a sensor comprised of fluorescent surrogates with attachment to covalently immobilized antibodies. Fluorescence signal loss was seen upon live E. coli O157:H7 challenge. The ‘acceleration’ in signal decay detected a spiking event after 7 minutes with a detection threshold of 100 colony forming units per milliliter. The sensor surface was stable for 300 sampling times over a 5 hour period. This sensor design is adaptable for integration into remote sensing type systems due to the small size, low power requirements and stability.

Keywords: Biosensor, Autonomous, E. coli, Remote sensing, Immunosensor

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1 Michael J. ANDERSON, 2 Andmorgan R. FISHER, 2 Alex LY, 1, 3 Evangelyn C. ALOCILJA and 2 Clint B. SMITH

¹ Michael J. ANDERSON, ²Andmorgan R. FISHER, ²Alex LY, ^{1, 3}Evangelyn C. ALOCILJA and ²Clint B. SMITH