Vol. 236, Issue 8, August 2019, pp. 28-39

Abstract: ATP concentrations affecting the anti-inflammatory and the pro- inflammatory status were conducted based on directly testing ATP concentration in the biological samples using organo-metallic devices. A heat-damaged biomimetic Matrix Metalloproteinase-2 (MMP-2) / Heat Shock Protein (HSP 60) Sensor 1 as a “Stressful” model was tested in the presence of 60 nM ATP using organic milk samples, and significant ATP extracellular hydrolysis curves were observed compared with the human milk samples, that raised the superconductivity at zero-bias. The reversible membrane potential (RMP) change in the presence of ATP from 100 aM to 800 nM was tested using a “Healthy” 3D-cage biomimetic MMP-2/ HSP60-like device 2, the RPM maintained constant with 1.3 % voltage increase rate compared with that of the ATP. Results of two levels of LPS challenges on the two types of milk samples in the presence of 60 nM ATP using Sensor 2, show organic milk vulnerable with 18.8 ± 0.3 % recoveries
at 90 fg/mL LPS compared 103 ± 0.7 % recovery for human milk samples.

Keywords: 3D-Cage Structure Membrane, Biomimetic MMP-2/HSP60 Multiple Functioning Device, ATP Quantitation, Biological Specimens, Reversible Membrane Potential (RMP), Ratio of Action/Resting potential.

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